Normative metrics of the brain radiodensity histogram derived from routine clinical head CT images can be used to develop a model of normal brain development.
Surprisingly, minocycline induced widespread cortical apoptosis and exacerbated MKtriggered cell death. In some areas such as the subiculum, the pro-apoptotic effect of minocycline was even more pronounced than that elicited by MK These data reveal among antipsychotics unique pro-apoptotic properties of minocycline, raising concerns regarding consequences for brain development and the use in children. Recent investigations of childhood neurodevelopmental and neurodegenerative disorders highlight the underlying genetic programming and experience-dependent remodeling of neural circuitry.
Recent neuroimaging and molecular biological studies on postnatal brain growth disorders have broadened our view of both typical and pathological postnatal neurodevelopment. In the adult brainastrocytes are known to intimately ensheath blood vessels and actively coordinate local neural activity and blood flow. We used in situ hybridization to map klotho mRNA expression in the developing and adult rat brain and report moderate, widespread expression across grey matter regions.
Normative curves were fitted by polynomial regression analysis. Histogram analysis was performed on brain -extracted images, and histogram mean, mode, full width at half maximum, skewness, kurtosis, and SD were correlated with subject age. These show a novel oligodendrocyte function expressed during early postnatal brain development, where these cells participate in the formation of cerebellar circuitries, and influence its development.
Lastly, we find that blood vessel morphology in mutant cortices recovers substantially at later stages, following astrogliosis. Correlating gene and protein function with brain growth trajectories uncovers postnatal biological mechanisms, including neuronal arborization, synaptogenesis and pruning, and gliogenesis and myelination. Undernourished mice given glutamine showed increased CA1 layer volume as compared with the other groups, consistent with the trend toward increased of neurons.
These provide the first anatomical localization of klotho mRNA and protein in rat brain parenchyma and demonstrate that klotho levels vary during early postnatal development. Zinc serum and brain levels and hippocampal neurotransmitters were also evaluated.
Participants comprised infants born at brain injury seen on cranial ultrasonography or congenital or chromosomal abnormalities were excluded. Average total brain volume was cm 3 at birth, cm 3 at 1 year, and cm 3 at 2 years.
Fetal magnetoencephalography fMEG is the only non-invasive method for investigating evoked brain responses and spontaneous brain activity generated by the fetus "in utero". Myelin ensures the fast conduction of the nerve impulse; in the adult, myelin proteins have an inhibitory role on axon growth and regeneration after injury.
Summary In order to understand typical and abnormal postnatal brain development, clinicians and researchers should characterize brain growth trajectories in the context of neurogenetic syndromes.
Furthermore, concomitant with loss of astroglial interactions, we find increased endothelial cell proliferation, enlarged vessel luminal size as well as enhanced cytoskeletal gene expression in pericytes, which suggests compensatory changes in vascular cells. Understanding mechanisms and trajectories of postnatal brain growth will aid in differentiating, diagnosing, and potentially treating neurodevelopmental disorders.
Brain growth in the early postnatal period following preterm birth has not been well described. We observed an impressive deregulation in the expression of molecules involved in axon growth, guidance and synaptic plasticity. This study of infants born at brain injury aimed to accomplish the following: 1 assess the reproducibility of linear measures made from cranial ultrasonography, 2 evaluate brain growth using sequential cranial ultrasonography linear measures from birth to term-equivalent age, and 3 explore perinatal predictors of postnatal brain growth.
Several genes have also been implicated in retinal astrocytes for regulating vessel development. Direct segmentation of CT images showed that changes in brain radiodensity histogram skewness correlated with, and can be explained by, a relative increase in gray matter volume and an increase in gray and white matter tissue density that occurs during this period of brain maturation. Fetal auditory as well as visual-evoked fields have been successfully recorded in basic stimulus-response studies.
Brain zinc levels were increased in the nourished and undernourished-glutamine treated mice as compared to the undernourished controls on day 7. One hundred twenty consecutive head CTs with normal findings meeting the inclusion criteria from children from birth to 2 years were retrospectively identified from 3 different CT scan platforms.
Moreover, paradigms investigating precursors for cognitive….
The histogram kurtosis also decreased in the first days to approach a normal distribution. Synaptophysin and myelin basic protein brain expressions were evaluated by immunoblot. To determine the roles that astrocytes may play during brain angiogenesis, we employ genetic approaches to inhibit astrogliogenesis during perinatal corticogenesis and examine its effects on brain vessel development. Reports vary on where klotho is expressed within the brain parenchyma, and no data is available as to whether klotho levels change across postnatal development. Oligodendrocyte ablation modifies localization and function of ionotropic glutamate receptors in Purkinje neurons.
The broad-spectrum antibiotic minocycline elicits antipsychotic and neuroprotective effects. Astroglia are a major cell type in the brain and play a key role in many aspects of brain development and function.
NMDA receptor NMDAR antagonists induce in perinatal rodent cortical apoptosis and protracted schizophrenia-like alterations ameliorated by antipsychotic treatment. Klotho protein co-localized with both the neuronal marker NeuN, as well as, oligodendrocyte marker olig2. Brain tissue and fluid spaces were measured from cranial ultrasonography performed as part of routine clinical care. Myelination occurs during the first postnatal weeks and, in rodents, is completed during the third week after birth.
Immunohistochemistry revealed a protein expression pattern similar to the mRNAwith klotho protein expressed widely throughout the brain. Copyright Elsevier Inc. All rights reserved. Expression of klotho mRNA and protein in rat brain parenchyma from early postnatal development into adulthood.
Klotho is most abundant in kidney and expressed in a limited of other organs, including the brainwhere klotho levels are highest in choroid plexus. Genetic control of postnatal human brain growth.
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Purpose of review Studies investigating postnatal brain growth disorders inform the biology underlying the development of human brain circuitry. A functional requirement for astroglia in promoting blood vessel development in the early postnatal brain. These effects were accompanied by an outstanding increase of neurofilament staining observed 4 hours after the beginning of the ablation protocol, likely dependent from sprouting of cerebellar fibers. Zinc and glutamine improve brain development in suckling mice subjected to early postnatal malnutrition. The effect of zinc and glutamine on brain development was investigated during the lactation period in Swiss mice.
Undernourished glutamine-treated mice showed increased hippocampal gamma-aminobutyric acid and synaptophysin levels on day We conclude that glutamine or zinc protects against malnutrition-induced brain developmental impairments. In this article, we addressed this question by transgenically targeted ablation of proliferating oligodendrocytes during cerebellum development. The effects of scan platform were investigated.
During development of the neural retina, blood vessel growth follows a meshwork of astrocytic processes. Conversely, overexpression of klotho extends mouse lifespan. PubMed Central. Minocycline exacerbates apoptotic neurodegeneration induced by the NMDA receptor antagonist MK in the early postnatal mouse brain.
We find that conditional deletion from glial progenitors of orc3, a gene required for DNA replication, dramatically reduces glial progenitor cell in the subventricular zone and astrocytes in the early postnatal cerebral cortex.
This suggests a role of astrocytes in promoting angiogenesis throughout the central nervous system. Oligodendrocytes are the glial cells responsible for myelin formation.
Recent findings Clinically, brain growth disorders are heralded by diverging head size for a given age and sex, but are more precisely characterized by brain imaging, postmortem analysis, and animal model studies. During brain development, oligodendrocytes precursors originating in multiple locations along the antero-posterior axis actively proliferate and migrate to colonize the whole brain. Clinton, Sarah M. Without the age-regulating protein klotho, mouse lifespan is shortened and the rapid onset of age-related disorders occurs.
Whether the initial interactions between oligodendrocytes and neurons might play a functional role before the onset of myelination is still not completely elucidated. Consistent with a delayed growth but not regression of vessels, we find neither ificant net decreases in vessel density between different stages after normalizing for cortical expansion nor obvious apoptosis of endothelial cells in these mutants.
These thus implicate a functional requirement for astroglia in promoting blood vessel growth during brain development. Interestingly, we show that depletion of oligodendrocytes at postnatal day 1 P1 profoundly affects the establishment of cerebellar circuitries. The majority of brain growth and development occur in the first 2 years of life.
In addition, zinc supplementation improved cliff avoidance and head position during swim behaviors especially on days 9 and Using de-based stereological methods, we found a ificant increase in the volume of CA1 neuronal cells in undernourished control mice, which was not seen in mice receiving zinc or glutamine alone or in combination.
Early-life stress origins of gastrointestinal disease: animal models, intestinal pathophysiology, and translational implications
This study investigated these changes by analysis of the brain radiodensity histogram of head CT scans from the clinical population, years of age. Brain radiodensity histogram skewness was positive at birth, declining logarithmically in the first days of life. Undernourished groups received daily supplementation with glutamine by subcutaneous injections starting at day 2 and continuing until day Glutamine mM, microL was used for morphological and behavioral studies. This research is becoming increasingly important for the diagnosis and treatment of childhood neurodevelopmental disorders, including autism and related disorders.
Here we review recent research on typical and abnormal postnatal brain growth and examine potential biological mechanisms. Zinc with or without glutamine improved weight gain as compared to untreated, undernourished controls. This, in turn, in severe reductions in both the density and branching frequency of cortical blood vessels.